| Audio | Dolby Digital 5.1, Dolby Surround |
| Subtitles | English, Spanish |
| Languages | English, Spanish |
| Aspect Ratio | 2.40:1 |
| Color | Color |
| Video Standard | NTSC |
| Packaging | Keep case, Slipcase, Other (48-page “Production Notes” booklet) |
| Classification | MPAA PG-13 |
| Catalog Number | 23535 |
| Barcode | 0 43396 23535 9 |
| ISBN | 1-4248-9418-2 |
| Catalog Number | 22597 |
| Barcode | 0 43396 22597 8 |
| ISBN | 1-4248-8406-3 |
| Part Number (Slipcase) | 23535SLP |
| Part Number (Slipcase) | 22597ORN |
| Part Number (Case) | 22597LIT |
| Part Number (Bonus Disc Holder) | 10059 EnvyPak™ |
| Part Number (Insert) | 10077212 |
| Part Number (Book) | 10077134 |
| Matrix Layer 0 | DVDL-515860A1 1 |
| Matrix Layer 1 | DVDL-515860B1 1 |
| Mastering SID Code Layer 0 | IFPI L333 |
| Mastering SID Code Layer 1 | IFPI L333 |
| Mould SID Code Read Side | IFPI QW48 |
| Mould SID Code Label Side | IFPI QW49 |
| Stamper Layer 0 | A15 |
| Stamper Layer 1 | A08 |
| Matrix Layer 0 | DVDL-515870A1 5 |
| Matrix Layer 1 | DVDL-515870B1 1 |
| Mastering SID Code Layer 0 | IFPI L333 |
| Mastering SID Code Layer 1 | IFPI L333 |
| Mould SID Code Read Side | IFPI QW78 |
| Mould SID Code Label Side | IFPI QW79 |
| Stamper Layer 0 | A01 |
| Stamper Layer 1 | A03 |
| Matrix | DVSS-523160A1 1 |
| Mastering SID Code | IFPI L331 |
| Mould SID Code Read Side | IFPI QW2C |
| Mould SID Code Label Side | IFPI QW2D |
| Stamper | A02 |
| Brand | Columbia Pictures, Sony Pictures Home Entertainment, Marvel |
| Territory | United States, Canada |
| Notes | Extras:
Disc 1:
- Film Maker and Cast Commentary Tracks.
- Bloopers
- Snow Patrol Music Video
- Photo Galleries
Disc 2:
- Grains Of Sand - Building Sandman
- Re-imaging The Goblin
- Covered In Black – Creating Venom
- On Location In New York and Cleveland
- Inside the Editing Room & Science of Sound
- 3 Stunts Featurettes
- More
Bonus DVD:
- On The Set: Spider-Man 3
- The Spider-Man 3 Spin Your Own Webisode Contest, sponsored by Target
- The 5 Winning Webisodes
- The Winners at the Award Ceremony held at the LA Film Festival |